Analysis Note

ControlJurkat nuclear extract, A-431 whole cell lysate

Application

Research Sub CategoryTranscription Factors

Research CategoryEpigenetics & Nuclear Function

Anti-CREB Antibody is a Mouse Monoclonal Antibody for detection of CREB also known as cAMP-response Element Binding Protein & has been validated in ELISA & WB.

Western blot: 1-5 µg/mL. Recognizes the ~43kDa protein.

ELISA: 0.1-1.0 µg/mL

Optimal working dilutions must be determined by end user.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

CREB is a beta ZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth and neuronal differentiation in certain neuronal populations. CREB promotes outgrowth and differentiation as a mediator of Neurotrophin pathways. CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+ and stress signaling.

Immunogen

Recombinant CREB-A protein.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: 04-218

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified immunoglobulin. Liquid in PBS containing 0.1% sodium azide.

Format: Purified

Specificity

Reacts with CREB.

Storage and Stability

Maintain at 2-8°C in undiluted aliquots for up to 6 months after date of receipt.